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trypsin edta solution (Nacalai)

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Nacalai trypsin edta solution
Trypsin Edta Solution, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trypsin+solution/edta+trypsin/pm42268311-44-9-11
Average 86 stars, based on 1 article reviews

trypsin edta solution - by Bioz Stars, 2026-10

86/100 stars

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Incubation:

Article Title: Harnessing synaptic vesicle release and recycling with antibody shuttle for targeted delivery of therapeutics to neurons
Article Snippet: .. In each experiment, cells were washed with PBS (WAKO) after incubation and collected in 0.125% trypsin solution (NACALAI TESQUE). ..

Article Title: RNA Sequencing Reveals Effects of a New Complex Bath Additive on Cultured Cells
Article Snippet: .. After 24 h of incubation, the cells were washed with 2 mL of PBS; after which, 400 μL/well of trypsin solution (2.5 g/L trypsin and 1 mmol/L EDTA solution, Cat#32777-44; Nacalai Tesque, Kyoto, Japan) was added to detach the cells, followed by incubation at 37 ◦C for 5 min. ..

Article Title: Extraction and Characterization of β-Glucan from Kombucha Bacterial Cellulose and Concentrated Kombucha: Evaluating their Bile Acid Binding Capacity
Article Snippet: .. A trypsin solution (Nacalai tesque, Japan) was added (at the final concentration of 1 % (v/v)) to digest proteins, and the mixture was incubated at 37°C for 30 min. After incubation, the extract solution was centrifuged, and the collected supernatant was precipitated with 2 volumes of ethanol at 4°C overnight to obtain β-glucan [16]. .. Finally, the pellet was collected by centrifugation, resuspended in distilled water, and dialyzed (dialysis bag, cut off: 3.5 KDa) at room temperature for 24 h. The resulting suspension was then centrifuged, and the pellet was dried using freeze-drying (FDU-2200, EYELA, Japan) and stored at room temperature [17].

other:

Article Title: Harnessing Synaptic Vesicle Release and Recycling with Antibody Shuttle for Targeted Delivery of Therapeutics to Neurons
Article Snippet: In each experiment, cells 665 were washed with PBS (WAKO) after incubation and collected in 0.125% Trypsin 666 Solution (NACALAI TESQUE).

Inhibition:

Article Title: PIKFYVE inhibition induces endosome- and lysosome-derived vacuole enlargement via ammonium accumulation.
Article Snippet: .. After PIKFYVE inhibition, cells were detached with 250 μL trypsin solution (Nacalai, 32777- 44) and collected in 1.5 mL tubes with 1 mL culture medium. ..

Centrifugation:

Article Title: MCPyV small T antigen enhances HPV16 oncogene expression, promotes Ca Ski cell proliferation, and reduces 5-fluorouracil-induced apoptosis in cervical cancer cells
Article Snippet: .. To detach them from the plate, 250 μL of trypsin solution (Nacalai Tesque, Kyoto, Japan) was applied, followed by centrifugation at 350 × g for 5 minutes to collect the cells. ..

Concentration Assay:

Irradiation:

Article Title: The COX-2/PGE2 Response Pathway Upregulates Radioresistance in A549 Human Lung Cancer Cells through Radiation-Induced Bystander Signaling
Article Snippet: .. Immediately after X-ray irradiation, the irradiated cells and controls were harvested using 2.5 g/L trypsin solution (Nacarai Tesque Inc., Kyoto, Japan) and plated in triplicate to obtain approximately 200 surviving cells per 10-cm dish (BD Falcon, MA, USA). ..